KLF10 Polyclonal antibody

KLF10 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, Chip, ELISA

Conjugate

Unconjugated

Cat no : 11881-1-AP

Synonyms

EGR alpha, EGRA, KLF10, Krueppel like factor 10, Kruppel like factor 10, TIEG, TIEG 1, TIEG1



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11881-1-AP targets KLF10 in WB, IP, IHC, IF, Chip, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF10 fusion protein Ag2482
Full Name Kruppel-like factor 10
Calculated Molecular Weight 469 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC011538
Gene Symbol KLF10
Gene ID (NCBI) 7071
RRIDAB_2877801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF10 (Krueppel-like factor 10), also known as TIEG1, is a transcription factor that plays a role in the regulation of cell growth. It binds to the consensus sequence 5'-GGTGTG-3'. KLF10 plays a role in the response to TGFbeta (through the TGFbeta/Smad signalling pathway) and estrogen. In both cases, KLF10 induces or repressed gene transcription to enhance the TGFbeta/Smad signalling pathway, as well as other pathways, to regulate cell proliferation, differentiation and apoptosis. KLF10 has been shown to have a role in skeletal disease (osteopenia/osteoporosis), hypertrophic cardiomyopathy, and breast and prostate cancer.This antibody is a rabbit polyclonal antibody raised against a full-length human KLF10 protein, and specifically recognizes the 52kd KLF10 protein.

Protocols

Product Specific Protocols
WB protocol for KLF10 antibody 11881-1-APDownload protocol
IF protocol for KLF10 antibody 11881-1-APDownload protocol
IP protocol for KLF10 antibody 11881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanChip

Sci Rep

Polymorphism rs7278468 is associated with Age-related cataract through decreasing transcriptional activity of the CRYAA promoter.

Authors - Xiaoyin Ma
humanIF

J Mol Cell Cardiol

Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.

Authors - Yongyun Li
humanWB,IHC

Acta Histochem

KLF10 is upregulated in osteoarthritis and inhibits chondrocyte proliferation and migration by upregulating Acvr1 and suppressing inhbb expression.

Authors - Langlang Zheng
mouse,canineIF

Cell Death Discov

Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis

Authors - Yang Zhang
mouse,humanWB

Cell Signal

Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis

Authors - Xiaoyan Chen