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KLRG1 Polyclonal antibody

KLRG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 10974-1-AP

Synonyms

2F1, CLEC15A, KLRG1, MAFA, MAFA 2F1, MAFA L, MAFA LIKE, MAFA like receptor, MAFAL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLRG1 Polyclonal antibody (10974-1-AP)

    WB analysis using 10974-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood leukocyte using KLRG1 Polyclonal antibody (10974-1-AP)

    WB analysis of human peripheral blood leukocyte using 10974-1-AP

    Human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using KLRG1 Polyclonal antibody (10974-1-AP)

    WB analysis of Raji using 10974-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KLRG1 Polyclonal antibody (10974-1-AP)

    IHC staining of human breast cancer using 10974-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KLRG1 Polyclonal antibody (10974-1-AP)

    IHC staining of human breast cancer using 10974-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, Raji cells, human peripheral blood leukocyte, NK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

10974-1-AP targets KLRG1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLRG1 fusion protein Ag1407
Full Name killer cell lectin-like receptor subfamily G, member 1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC012621
Gene Symbol KLRG1
Gene ID (NCBI) 10219
RRIDAB_2234383
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role on NK- and T-cell function (PMID: 19009530; 20373518).

Protocols

Product Specific Protocols
WB protocol for KLRG1 antibody 10974-1-APDownload protocol
IHC protocol for KLRG1 antibody 10974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Brain

Highly differentiated cytotoxic T cells in inclusion body myositis.

Authors - Steven A Greenberg
View Article
humanIF

J Neurol

Morphological and molecular comparison of HIV-associated and sporadic inclusion body myositis

Authors - Sinja Vogt
View Article