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Kininogen 1 Monoclonal antibody

Kininogen 1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

10E8D11

Cat no : 66123-1-Ig

Synonyms

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    WB analysis of human heart using 66123-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    WB analysis of human placenta using 66123-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    WB analysis of human plasma using 66123-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IHC staining of human breast cancer using 66123-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IHC staining of human breast cancer using 66123-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IHC staining of human ovary tumor using 66123-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IHC staining of human ovary tumor using 66123-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IF Staining of HeLa using 66123-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Kininogen 1 antibody (66123-1-Ig, Clone: 10E8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman heart tissue, human placenta tissue, human plasma tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66123-1-Ig is Kininogen 1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Kininogen 1 fusion protein Ag19233
Full Name kininogen 1
Calculated Molecular Weight 644 aa, 72 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC060039
Gene Symbol KNG1
Gene ID (NCBI) 3827
RRIDAB_2881522
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

Protocols

Product Specific Protocols
WB protocol for Kininogen 1 antibody 66123-1-IgDownload protocol
IHC protocol for Kininogen 1 antibody 66123-1-IgDownload protocol
IF protocol for Kininogen 1 antibody 66123-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols