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LAIR1 Monoclonal antibody

LAIR1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A5D1

Cat no : 67220-1-Ig

Synonyms

CD305, hLAIR1, LAIR 1, LAIR1

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using LAIR1 Monoclonal antibody (67220-1-Ig)

    WB analysis of THP-1 using 67220-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using LAIR1 Monoclonal antibody (67220-1-Ig)

    WB analysis of HL-60 using 67220-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of TF-1 cells using LAIR1 Monoclonal antibody (67220-1-Ig)

    WB analysis of TF-1 using 67220-1-Ig

    TF-1 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LAIR1 Monoclonal antibody (67220-1-Ig)

    IHC staining of human tonsillitis using 67220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67220-1-Ig (LAIR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LAIR1 Monoclonal antibody (67220-1-Ig)

    IHC staining of human tonsillitis using 67220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67220-1-Ig (LAIR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LAIR1 Monoclonal antibody (67220-1-Ig)

    IF Staining of human tonsillitis using 67220-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LAIR1 Monoclonal antibody (67220-1-Ig)

    IF Staining of human tonsillitis using 67220-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells, TF-1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67220-1-Ig targets LAIR1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LAIR1 fusion protein Ag14459
Full Name leukocyte-associated immunoglobulin-like receptor 1
Calculated Molecular Weight 287 aa, 31 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC027899
Gene Symbol LAIR1
Gene ID (NCBI) 3903
RRIDAB_2882511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).

Protocols

Product Specific Protocols
WB protocol for LAIR1 antibody 67220-1-IgDownload protocol
IHC protocol for LAIR1 antibody 67220-1-IgDownload protocol
IF protocol for LAIR1 antibody 67220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cancer Med

Development and validation of a leukocyte-associated immunoglobulin-like receptor-1 prognostic signature for lower-grade gliomas.

Authors - Zhansheng Fang
View Article