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LCK Monoclonal antibody

LCK Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2D10C2

Cat no : 60162-1-Ig

Synonyms

LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16

Validation Data Gallery

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  • Western Blot (WB) analysis of pig thymus tissue using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of pig thymus using 60162-1-Ig

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat thymus tissue using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of rat thymus using 60162-1-Ig

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of Jurkat using 60162-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using LCK Monoclonal antibody (60162-1-Ig)

    WB analysis of Ramos using 60162-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human tonsillitis using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human tonsillitis using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human lymphoma using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using LCK Monoclonal antibody (60162-1-Ig)

    IHC staining of human lymphoma using 60162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of human tonsillitis using 60162-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of human tonsillitis using 60162-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LCK Monoclonal antibody (60162-1-Ig)

    IF Staining of HepG2 using 60162-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using LCK Monoclonal antibody (60162-1-Ig)

    FC experiment of HepG2 using 60162-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inpig thymus tissue, Ramos cells, rat thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60162-1-Ig targets LCK in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LCK fusion protein Ag7694
Full Name lymphocyte-specific protein tyrosine kinase
Calculated Molecular Weight 539 aa, 56 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC013200
Gene Symbol LCK
Gene ID (NCBI) 3932
RRIDAB_10663101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for LCK antibody 60162-1-IgDownload protocol
IHC protocol for LCK antibody 60162-1-IgDownload protocol
IF protocol for LCK antibody 60162-1-IgDownload protocol
FC protocol for LCK antibody 60162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Gen Med

Identification of Biomarkers Related to Regulatory T Cell Infiltration in Oral Squamous Cell Carcinoma Based on Integrated Bioinformatics Analysis.

Authors - Chao Wang
View Article