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Galectin 2 Polyclonal antibody

Galectin 2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12849-2-AP

Synonyms

Gal 2, Galectin 2, HL14, Lactose binding lectin 2, LGALS2, S Lac lectin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Galectin 2 Polyclonal antibody (12849-2-AP)

    WB analysis using 12849-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12849-2-AP (Galectin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Galectin 2 Polyclonal antibody (12849-2-AP)

    IHC staining of human colon cancer using 12849-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12849-2-AP (Galectin 2 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inmouse small intestine tissue, rat small intestine tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12849-2-AP targets Galectin 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin 2 fusion protein Ag3979
Full Name lectin, galactoside-binding, soluble, 2
Calculated Molecular Weight 132 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC029063
Gene Symbol LGALS2
Gene ID (NCBI) 3957
RRIDAB_2136753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Galectin 2 antibody 12849-2-APDownload protocol
IHC protocol for Galectin 2 antibody 12849-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin.

Authors - Maria Claudia Vladoiu
View Article
humanWB,IHC

J Gastroenterol

Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer.

Authors - Jung Ji-Han JH
View Article
humanIHC

Oncotarget

Galectin signatures contribute to the heterogeneity of breast cancer and provide new prognostic information and therapeutic targets.

Authors - Andrée-Anne Grosset
View Article