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LGI3 Polyclonal antibody

LGI3 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 21919-1-AP

Synonyms

LGI1 like protein 4, LGI3, LGIL4

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis of HepG2 using 21919-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis of mouse lung using 21919-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis of A549 using 21919-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis using 21919-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis of mouse liver using 21919-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human lung using 21919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human lung using 21919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human lung using 21919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human brain using 21919-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human brain using 21919-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human lung using 21919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LGI3 Polyclonal antibody (21919-1-AP)

    IF Staining of HepG2 using 21919-1-AP

    Immunofluorescent analysis of HepG2 cells using 21919-1-AP (LGI3 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-Rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using LGI3 Polyclonal antibody (21919-1-AP)

    FC experiment of HepG2 using 21919-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug LGI3 antibody (21919-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHepG2 cells, mouse lung tissue, A549 cells, mouse liver tissue, SMMC-7721 cells
Positive IHC detected inhuman lung tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21919-1-AP is LGI3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGI3 fusion protein Ag16257
Full Name leucine-rich repeat LGI family, member 3
Calculated Molecular Weight 548 aa, 62 kDa
Observed Molecular Weight 55-62 kDa
GenBank Accession NumberBC142997
Gene Symbol LGI3
Gene ID (NCBI) 203190
RRIDAB_10838807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LGI3 antibody 21919-1-APDownload protocol
IHC protocol for LGI3 antibody 21919-1-APDownload protocol
IF protocol for LGI3 antibody 21919-1-APDownload protocol
FC protocol for LGI3 antibody 21919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols