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- KD/KO Validated
LGTN Polyclonal antibody
LGTN Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12840-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, PC-3 cells |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
12840-1-AP targets LGTN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LGTN fusion protein Ag3579 |
| Full Name | ligatin |
| Calculated Molecular Weight | 584 aa, 65 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC001585 |
| Gene Symbol | LGTN |
| Gene ID (NCBI) | 1939 |
| RRID | AB_2135302 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LGTN antibody 12840-1-AP | Download protocol |
| IF protocol for LGTN antibody 12840-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.
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Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. | ||
Genes Dev Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling. | ||
Nat Commun A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.
| ||
Nucleic Acids Res Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.
| ||
