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  • Featured Product
  • KD/KO Validated

LHCGR Polyclonal antibody

LHCGR Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26424-1-AP

Synonyms

LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing hormone receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using LHCGR Polyclonal antibody (26424-1-AP)

    WB analysis of mouse ovary using 26424-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 26424-1-AP (LHCGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using LHCGR Polyclonal antibody (26424-1-AP)

    IHC staining of human thyroid cancer using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using LHCGR Polyclonal antibody (26424-1-AP)

    IHC staining of human thyroid cancer using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LHCGR Polyclonal antibody (26424-1-AP)

    IHC staining of human ovary using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LHCGR Polyclonal antibody (26424-1-AP)

    IHC staining of human ovary using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using LHCGR Polyclonal antibody (26424-1-AP)

    IHC staining of mouse ovary using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using LHCGR Polyclonal antibody (26424-1-AP)

    IHC staining of mouse ovary using 26424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 26424-1-AP (LHCGR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse ovary tissue using LHCGR Polyclonal antibody (26424-1-AP)

    IF Staining of mouse ovary using 26424-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using LHCGR antibody (26424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using LHCGR Polyclonal antibody (26424-1-AP)

    FC experiment of Jurkat using 26424-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LHCGR (26424-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse ovary tissue
Positive IHC detected inhuman thyroid cancer tissue, human ovary tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse ovary tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26424-1-AP targets LHCGR in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LHCGR fusion protein Ag19259
Full Name LHCGR
Calculated Molecular Weight 699 aa, 79 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC157028
Gene Symbol LHCGR
Gene ID (NCBI) 3973
RRIDAB_2880511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LHCGR, also known as LHR or LSHR, belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Protocols

Product Specific Protocols
WB protocol for LHCGR antibody 26424-1-APDownload protocol
IHC protocol for LHCGR antibody 26424-1-APDownload protocol
IF protocol for LHCGR antibody 26424-1-APDownload protocol
FC protocol for LHCGR antibody 26424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Biochem Biophys Res Commun

Effects of hCG on DA neuronal death of Parkinson's disease

Authors - Shi-Min Wang
  • KD Validated
View Article