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  • Featured Product
  • KD/KO Validated

LIFR Polyclonal antibody

LIFR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22779-1-AP

Synonyms

CD118, LIF R, LIF receptor, LIFR, SJS2, STWS, SWS

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using LIFR Polyclonal antibody (22779-1-AP)

    WB analysis of human skeletal muscle using 22779-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using LIFR Polyclonal antibody (22779-1-AP)

    WB analysis of mouse skeletal muscle using 22779-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using LIFR Polyclonal antibody (22779-1-AP)

    IP experiment of mouse skeletal muscle using 22779-1-AP

    IP result of anti-LIFR (IP:22779-1-AP, 4ug; Detection:22779-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LIFR Polyclonal antibody (22779-1-AP)

    IHC staining of human skeletal muscle using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using LIFR Polyclonal antibody (22779-1-AP)

    IHC staining of mouse cerebellum using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using LIFR Polyclonal antibody (22779-1-AP)

    IHC staining of mouse cerebellum using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LIFR Polyclonal antibody (22779-1-AP)

    IHC staining of human kidney using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LIFR Polyclonal antibody (22779-1-AP)

    IHC staining of human kidney using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using LIFR Polyclonal antibody (22779-1-AP)

    IHC staining of human placenta using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using LIFR Polyclonal antibody (22779-1-AP)

    IHC staining of human placenta using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LIFR Polyclonal antibody (22779-1-AP)

    IF Staining of HeLa using 22779-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22779-1-AP (LIFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using LIFR Polyclonal antibody (22779-1-AP)

    FC experiment of HeLa using 22779-1-AP

    1X10^6 HeLa cells were stained with .2ug LIFR antibody (22779-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human placenta tissue, mouse cerebellum tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22779-1-AP targets LIFR in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIFR fusion protein Ag18742
Full Name leukemia inhibitory factor receptor alpha
Calculated Molecular Weight 1097 aa, 124 kDa
Observed Molecular Weight 190 kDa
GenBank Accession NumberBC153096
Gene Symbol LIFR
Gene ID (NCBI) 3977
RRIDAB_2879165
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIFR, also known as CD118, is a subunit of a receptor for leukemia inhibitory factor (LIF). LIF is a pleiotropic cytokine of the interleukin-6 family which affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of LIF. The high-affinity complex also binds a related cytokine, oncostatin M (PMID: 8999038). LIFR has also been identified as a breast cancer metastasis suppressor that functions through the HIPPO-YAP pathway (PMID: 23001183).

Protocols

Product Specific Protocols
WB protocol for LIFR antibody 22779-1-APDownload protocol
IHC protocol for LIFR antibody 22779-1-APDownload protocol
IF protocol for LIFR antibody 22779-1-APDownload protocol
IP protocol for LIFR antibody 22779-1-APDownload protocol
FC protocol for LIFR antibody 22779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Nature

Exercise plasma boosts memory and dampens brain inflammation via clusterin.

Authors - Zurine De Miguel
View Article
mouseWB

Nat Commun

A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.

Authors - Fan Yao
  • KO Validated
View Article
humanWB

Clin Transl Med

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Authors - An-Dong Liu
View Article
humanWB,IHC,IF

Hum Mol Genet

Mutations in the leukemia inhibitory factor receptor (LIFR) gene and Lifr deficiency cause urinary tract malformations.

Authors - Anne Kosfeld
View Article
humanWB

J Exp Clin Cancer Res

miR-589 promotes gastric cancer aggressiveness by a LIFR-PI3K/AKT-c-Jun regulatory feedback loop.

Authors - Feifei Zhang
View Article
mouseWB

Biotechnol Bioeng

Enzyme-free cell detachment mediated by resonance vibration with temperature modulation.

Authors - Yuta Kurashina
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Clarisse (Verified Customer) (12-12-2022)

Low signal, regardless of the method of fixation.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human cerebral organoids