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LILRA5 Polyclonal antibody

LILRA5 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC,ELISA

Conjugate

Unconjugated

Cat no : 18821-1-AP

Synonyms

CD85, CD85F, ILT 11, ILT11, LILRA5, LILRB7, LIR 9, LIR9

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using LILRA5 Polyclonal antibody (18821-1-AP)

    WB analysis of K-562 using 18821-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18821-1-AP (LILRA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using LILRA5 Polyclonal antibody (18821-1-AP)

    WB analysis of K-562 using 18821-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18821-1-AP (LILRA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of K-562 cells using LILRA5 Polyclonal antibody (18821-1-AP)

    FC experiment of K-562 using 18821-1-AP

    1X10^6 K-562 cells were stained with 0.2ug LILRA5 antibody (18821-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inK-562 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18821-1-AP targets LILRA5 in WB, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5
Calculated Molecular Weight 299 aa, 33 kDa
Observed Molecular Weight 31-33 kDa
GenBank Accession NumberBC153203
Gene Symbol LILRA5
Gene ID (NCBI) 353514
RRIDAB_2281279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LILRA5, also named as ILT11, LILRB7, LIR9 and CD85f, may plays a role in triggering innate immune responses. It does not function in class I MHC antigens recognition. The antibody recognizes the isoform 1 and isoform 2 of LILRA5.

Protocols

Product Specific Protocols
WB protocol for LILRA5 antibody 18821-1-APDownload protocol
FC protocol for LILRA5 antibody 18821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols