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LILRB1 Polyclonal antibody

LILRB1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 26455-1-AP

Synonyms

CD85, CD85J, ILT 2, ILT2, LILRB1, LIR 1, LIR1, MIR 7, MIR7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LILRB1 Polyclonal antibody (26455-1-AP)

    WB analysis using 26455-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26455-1-AP (LILRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using LILRB1 Polyclonal antibody (26455-1-AP)

    WB analysis of Ramos using 26455-1-AP

    Ramos cells were subjected to SDS PAGE followed by western blot with 26455-1-AP (LILRB1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using LILRB1 Polyclonal antibody (26455-1-AP)

    IHC staining of human liver using 26455-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26455-1-AP (LILRB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using LILRB1 Polyclonal antibody (26455-1-AP)

    IHC staining of human liver using 26455-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26455-1-AP (LILRB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LILRB1 Polyclonal antibody (26455-1-AP)

    IHC staining of human tonsillitis using 26455-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26455-1-AP (LILRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LILRB1 Polyclonal antibody (26455-1-AP)

    IHC staining of human tonsillitis using 26455-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26455-1-AP (LILRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Ramos cells using LILRB1 Polyclonal antibody (26455-1-AP)

    FC experiment of Ramos using 26455-1-AP

    1X10^6 Ramos cells were stained with 0.20 ug Anti-Human LILRB1 (26455-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

Tested Applications

Positive WB detected inDaudi cells, Ramos cells
Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

26455-1-AP targets LILRB1 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LILRB1 fusion protein Ag24795
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 and 110 kDa
GenBank Accession NumberBC015731
Gene Symbol LILRB1
Gene ID (NCBI) 10859
RRIDAB_2880518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LILRB1, also named as CD85J or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells. The predicted MW of LILRB1 is 71 kDa and 26455-1-AP antibody recognizes the 71 and 110 kDa which might be the noncovalently linked monomer. (PMID:17601702; 15474475; 17549736; 24098421)

Protocols

Product Specific Protocols
WB protocol for LILRB1 antibody 26455-1-APDownload protocol
IHC protocol for LILRB1 antibody 26455-1-APDownload protocol
FC protocol for LILRB1 antibody 26455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int Immunopharmacol

LILRB1+ immune cell infiltration identifies immunosuppressive microenvironment and dismal outcomes of patients with ovarian cancer

Authors - Xiaoyu Xu
View Article