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LIPA Polyclonal antibody

LIPA Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12956-1-AP

Synonyms

CESD, Cholesteryl esterase, LAL, LIPA, Lipase A, Sterol esterase



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, HEK-293 cells, HepG2 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12956-1-AP targets LIPA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIPA fusion protein Ag3627
Full Name lipase A, lysosomal acid, cholesterol esterase
Calculated Molecular Weight 399 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC012287
Gene Symbol LIPA
Gene ID (NCBI) 3988
RRIDAB_2078477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human acid lipase/cholesteryl esterase (LIPA) is a 46-kDa glycoprotein required for the lysosomal hydrolysis of cholesteryl esters and triglycerides that cells acquire through the receptor-mediated endocytosis of low-density lipoproteins(PMID:8432549). Defects in LIPA are the cause of Wolman disease (WOD)(PMID:8146180) and cholesteryl ester storage disease (CESD)(PMID:9633819). It has 2 isoforms produced by alternative splicing with the molecular weight of 45 kDa and 39 kDa. The full length protein has a signal peptide and several glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for LIPA antibody 12956-1-APDownload protocol
IHC protocol for LIPA antibody 12956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Commun (Lond)

Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.

Authors - Luwei He
ratWB

Phytomedicine

Morroniside, a novel GATA3 binding molecule, inhibits hepatic stellate cells activation by enhancing lysosomal acid lipase expression.

Authors - Lin An
ratWB

J Oncol

CircRNA-Associated ceRNA Network Reveals Focal Adhesion and Metabolism Pathways in Neuropathic Pain

Authors - Kun Wang
human,mouseWB,IHC,IF,IP

Cell Death Dis

Molecular and histological traits of reduced lysosomal acid lipase activity in the fatty liver.

Authors - Simone Carotti

Hepatol Commun

Evidence for a Novel Regulatory Interaction Involving Cyclin D1, Lipid Droplets, Lipolysis, and Cell Cycle Progression in Hepatocytes.

Authors - Heng Wu
mouseWB

Nutrients

Moderate Treadmill Exercise Alleviates NAFLD by Regulating the Biogenesis and Autophagy of Lipid Droplet

Authors - Yangjun Yang