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LMAN2 Polyclonal antibody

LMAN2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11496-1-AP

Synonyms

C5orf8, Glycoprotein GP36b, GP36B, Lectin mannose binding 2, lectin, mannose binding 2, LMAN2, VIP36

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LMAN2 Polyclonal antibody (11496-1-AP)

    WB analysis using 11496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11496-1-AP (LMAN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LMAN2 Polyclonal antibody (11496-1-AP)

    IHC staining of mouse testis using 11496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11496-1-AP (LMAN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LMAN2 Polyclonal antibody (11496-1-AP)

    IHC staining of mouse testis using 11496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11496-1-AP (LMAN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, rat heart tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11496-1-AP targets LMAN2 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMAN2 fusion protein Ag1966
Full Name lectin, mannose-binding 2
Calculated Molecular Weight 356 aa, 40 kDa
Observed Molecular Weight35-36 kDa
GenBank Accession NumberBC017263
Gene Symbol LMAN2
Gene ID (NCBI) 10960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LMAN2 antibody 11496-1-APDownload protocol
IHC protocol for LMAN2 antibody 11496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols