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  • Featured Product
  • KD/KO Validated

LONP2 Polyclonal antibody

LONP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18035-1-AP

Synonyms

lon peptidase 2, peroxisomal, Lon protease 2, Lon protease like protein 2, LONP, LONP2, LONPL, Peroxisomal Lon protease

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using LONP2 Polyclonal antibody (18035-1-AP)

    WB analysis of Jurkat using 18035-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18035-1-AP (LONP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LONP2 Polyclonal antibody (18035-1-AP)

    IHC staining of human prostate cancer using 18035-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18035-1-AP (LONP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LONP2 Polyclonal antibody (18035-1-AP)

    IHC staining of human prostate cancer using 18035-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18035-1-AP (LONP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LONP2 Polyclonal antibody (18035-1-AP)

    IF Staining of HepG2 using 18035-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18035-1-AP (LONP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18035-1-AP is LONP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LONP2 fusion protein Ag12650
Full Name lon peptidase 2, peroxisomal
Calculated Molecular Weight 852 aa, 95 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC093910
Gene Symbol LONP2
Gene ID (NCBI) 83752
RRIDAB_2878485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LONP2 antibody 18035-1-APDownload protocol
IHC protocol for LONP2 antibody 18035-1-APDownload protocol
IF protocol for LONP2 antibody 18035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Gene

Role of LONP2 in head and neck squamous cell carcinoma

Authors - Xiaobo Cui
  • KD Validated
View Article