• Featured Product
  • KD/KO Validated

LOX Polyclonal antibody

LOX Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17958-1-AP

Synonyms

LOX, lysyl oxidase, Protein lysine 6 oxidase



Tested Applications

Positive WB detected inJurkat cells, MKN-45 cells, SGC-7901 cells, NIH/3T3 cells, mouse skeletal muscle tissue, A375 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman stomach cancer tissue, mouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17958-1-AP targets LOX in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LOX fusion protein Ag12479
Full Name lysyl oxidase
Calculated Molecular Weight 417 aa, 47 kDa
Observed Molecular Weight 32 kDa, 45-47 kDa
GenBank Accession NumberBC074872
Gene Symbol LOX
Gene ID (NCBI) 4015
RRIDAB_2234541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LOX is a member of a multigene family (LOX, LOXL, LOXL2, LOXL3, and LOXL4). Lysyl oxidase (LOX), a copper-dependent enzyme, plays a critical role in the formation and repair of the extracellularmatrix (ECM) by catalyzing the crosslinking of elastin and collagen. LOX expression appears to be upregulated in malignancies and has recently been suggested to play a role in regulating tumor metastasis. It is synthesized as a 50 kDa proenzyme, secreted, and processed into an 32 kDa mature, active enzyme and an 18-kDa propeptide.

Protocols

Product Specific Protocols
WB protocol for LOX antibody 17958-1-APDownload protocol
IHC protocol for LOX antibody 17958-1-APDownload protocol
IP protocol for LOX antibody 17958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Syst

Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.

Authors - Stephanie A Zlatic
humanWB

Oncogene

Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids

Authors - Hui Zhao
humanIHC,IF

Oncogene

Cell-to-cell heterogeneity of EWSR1-FLI1 activity determines proliferation/migration choices in Ewing sarcoma cells.

Authors - G-A Franzetti
humanIHC

Int J Nanomedicine

Gallbladder Cancer Progression Is Reversed by Nanomaterial-Induced Photothermal Therapy in Combination with Chemotherapy and Autophagy Inhibition.

Authors - Qiang Cai
humanIHC

ACS Biomater Sci Eng

Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species.

Authors - Binghui Liu
humanIHC

Biomed Pharmacother

Drug resistance evaluation in novel 3D in vitro model.

Authors - Marta Nowacka