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  • Featured Product
  • KD/KO Validated

LRRC47 Polyclonal antibody

LRRC47 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23217-1-AP

Synonyms

KIAA1185, LRRC47

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using LRRC47 Polyclonal antibody (23217-1-AP)

    WB analysis of mouse liver using 23217-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using LRRC47 Polyclonal antibody (23217-1-AP)

    WB analysis of Neuro-2a using 23217-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using LRRC47 Polyclonal antibody (23217-1-AP)

    WB analysis of rat liver using 23217-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using LRRC47 Polyclonal antibody (23217-1-AP)

    WB analysis of NIH/3T3 using 23217-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using LRRC47 Polyclonal antibody (23217-1-AP)

    WB analysis of Neuro-2a using 23217-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using LRRC47 Polyclonal antibody (23217-1-AP)

    WB analysis of L02 using 23217-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LRRC47 Polyclonal antibody (23217-1-AP)

    IHC staining of human pancreas using 23217-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LRRC47 Polyclonal antibody (23217-1-AP)

    IHC staining of human pancreas using 23217-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using LRRC47 Polyclonal antibody (23217-1-AP)

    IF Staining of L02 using 23217-1-AP

    Immunofluorescent analysis of L02 cells using 23217-1-AP (LRRC47 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, L02 cells, Neuro-2a cells, NIH/3T3 cells, rat liver tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23217-1-AP is LRRC47 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC47 fusion protein Ag19736
Full Name leucine rich repeat containing 47
Calculated Molecular Weight 583 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC031301
Gene Symbol LRRC47
Gene ID (NCBI) 57470
RRIDAB_2879234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LRRC47 antibody 23217-1-APDownload protocol
IHC protocol for LRRC47 antibody 23217-1-APDownload protocol
IF protocol for LRRC47 antibody 23217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Structural basis for the final steps of human 40S ribosome maturation.

Authors - Michael Ameismeier
  • KD Validated
View Article
humanWB

bioRxiv

LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins

Authors - James A Saba
  • KD Validated
View Article
humanWB,CoIP,IHC

Cell Rep

The C9ORF72 repeat expansion alters neurodevelopment

Authors - Eric Hendricks
View Article