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LRRTM3 Polyclonal antibody

LRRTM3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27003-1-AP

Synonyms

LRRTM3

Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using LRRTM3 Polyclonal antibody (27003-1-AP)

    WB analysis of Neuro-2a using 27003-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27003-1-AP (LRRTM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LRRTM3 Polyclonal antibody (27003-1-AP)

    WB analysis of mouse brain using 27003-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27003-1-AP (LRRTM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using LRRTM3 Polyclonal antibody (27003-1-AP)

    WB analysis of rat brain using 27003-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 27003-1-AP (LRRTM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using LRRTM3 Polyclonal antibody (27003-1-AP)

    IHC staining of human brain using 27003-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27003-1-AP (LRRTM3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using LRRTM3 Polyclonal antibody (27003-1-AP)

    IHC staining of human brain using 27003-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27003-1-AP (LRRTM3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27003-1-AP is LRRTM3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRTM3 fusion protein Ag25452
Full Name leucine rich repeat transmembrane neuronal 3
Calculated Molecular Weight 581 aa, 66 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC111492
Gene Symbol LRRTM3
Gene ID (NCBI) 347731
RRIDAB_2880719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LRRTM3 antibody 27003-1-APDownload protocol
IHC protocol for LRRTM3 antibody 27003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols