LSM14A Polyclonal antibody

LSM14A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18336-1-AP

Synonyms

AlphaSNBP, C19orf13, FAM61A, hRAP55, hRAP55A, LSM14A, Protein FAM61A, Protein LSM14 homolog A, Protein SCD6 homolog, RAP55, RAP55A, RNA associated protein 55A



Tested Applications

Positive WB detected inA549 cells, HeLa cells, PC-3 cells, HEK-293T cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18336-1-AP targets LSM14A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSM14A fusion protein Ag12997
Full Name LSM14A, SCD6 homolog A (S. cerevisiae)
Calculated Molecular Weight 463 aa, 51 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC016842
Gene Symbol LSM14A
Gene ID (NCBI) 26065
RRIDAB_10644339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LSm14A was originally discovered to be a component of the P-bodies, it is a member of the LSm family of proteins, which have been shown to be involved in RNA processing events. The N terminus of LSm14A is a conserved LSm domain, whereas the C terminus contains two domains called "DFDF box" and "FDF_TFG box", respectively.

Protocols

Product Specific Protocols
WB protocol for LSM14A antibody 18336-1-APDownload protocol
IHC protocol for LSM14A antibody 18336-1-APDownload protocol
IF protocol for LSM14A antibody 18336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Elife

Selective sorting of microRNAs into exosomes by phase-separated YBX1 condensates.

Authors - Xiao-Man Liu
mouseIF

Cell Rep

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation

Authors - Nicolas Curdy
humanIF

Adv Sci (Weinh)

Oleic Acid Dissolves cGAS-DNA Phase Separation to Inhibit Immune Surveillance

Authors - Lina Wang
humanIF

Nucleic Acids Res

RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules

Authors - Vladimir Majerciak