LSS Polyclonal antibody

Name: LSS Polyclonal antibody
Brand: Proteintech
SKU: 13715-1-AP

LSS Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13715-1-AP

Synonyms

hOSC, Lanosterol synthase, LSS, OSC

Validation Data Gallery

Filter:

AllWBIPIHCIFFC

WB analysis using 13715-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13715-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13715-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13715-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13715-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13715-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 13715-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 13715-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13715-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis using 13715-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13715-1-AP (LSS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 13715-1-AP
IP result of anti-LSS (IP:13715-1-AP, 3ug; Detection:13715-1-AP 1:500) with HepG2 cells lysate 2900ug.
IHC staining of human liver using 13715-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 13715-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse testis using 13715-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse testis using 13715-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13715-1-AP (LSS Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 13715-1-AP
Immunofluorescent analysis of HepG2 cells using 13715-1-AP (LSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 13715-1-AP
1X10^6 HeLa cells were stained with 0.2ug LSS antibody (13715-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Cat No. 13715-1-AP

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Tested Applications

Positive WB detected in	HT-1376 cells, PC-3 cells, HeLa cells, HepG2 cells, mouse testis tissue, human brain tissue, mouse pancreas tissue, mouse liver tissue, SH-SY5Y cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:20-1:200
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

13715-1-AP targets LSS in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LSS fusion protein Ag4655
Full Name	lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
Calculated Molecular Weight	732 aa, 83 kDa
Observed Molecular Weight	70-75 kDa
GenBank Accession Number	BC035638
Gene Symbol	LSS
Gene ID (NCBI)	4047
RRID	AB_10597096
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.

Protocols

Product Specific Protocols
WB protocol for LSS antibody 13715-1-AP	Download protocol
IHC protocol for LSS antibody 13715-1-AP	Download protocol
IF protocol for LSS antibody 13715-1-AP	Download protocol
IP protocol for LSS antibody 13715-1-AP	Download protocol
FC protocol for LSS antibody 13715-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. Authors - Xiaohui Li View Article
human	WB	J Invest Dermatol Bi-allelic Variants in LSS Cause Palmoplantar Keratoderma-Congenital Alopecia Syndrome Type 2. Authors - Fang Yang View Article
human	WB,IHC	PLoS Genet Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice. Authors - Yoichi Wada View Article
rat	WB	J Biol Chem The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis. Authors - Lee G D Fryer View Article
human	WB, IHC	Phytomedicine Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression. Authors - Xing Yue Xu View Article
human	WB	J Biol Chem The antiviral enzyme viperin inhibits cholesterol biosynthesis. Authors - Timothy J Grunkemeyer View Article

LSS Polyclonal antibody

LSS Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 13715-1-AP

WB analysis of HeLa using 13715-1-AP

WB analysis of mouse liver using 13715-1-AP

WB analysis of HepG2 using 13715-1-AP

WB analysis of mouse testis using 13715-1-AP

WB analysis of human brain using 13715-1-AP

WB analysis of mouse pancreas using 13715-1-AP

WB analysis of PC-3 using 13715-1-AP

WB analysis of HeLa using 13715-1-AP

WB analysis using 13715-1-AP

IP experiment of HepG2 using 13715-1-AP

IHC staining of human liver using 13715-1-AP

IHC staining of human liver using 13715-1-AP

IHC staining of mouse testis using 13715-1-AP

IHC staining of mouse testis using 13715-1-AP

IF Staining of HepG2 using 13715-1-AP

FC experiment of HeLa using 13715-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Bi-allelic Variants in LSS Cause Palmoplantar Keratoderma-Congenital Alopecia Syndrome Type 2.

Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice.

The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.

Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.

The antiviral enzyme viperin inhibits cholesterol biosynthesis.