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LYN Polyclonal antibody

LYN Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18135-1-AP

Synonyms

JTK8, LYN, Tyrosine protein kinase Lyn

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LYN Polyclonal antibody (18135-1-AP)

    WB analysis of HeLa using 18135-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using LYN Polyclonal antibody (18135-1-AP)

    WB analysis of L02 using 18135-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using LYN Polyclonal antibody (18135-1-AP)

    WB analysis of HL-60 using 18135-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LYN Polyclonal antibody (18135-1-AP)

    WB analysis of Jurkat using 18135-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using LYN Polyclonal antibody (18135-1-AP)

    WB analysis of K-562 using 18135-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LYN Polyclonal antibody (18135-1-AP)

    IP experiment of HeLa using 18135-1-AP

    IP result of anti-LYN (IP:18135-1-AP, 4ug; Detection:18135-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LYN Polyclonal antibody (18135-1-AP)

    IHC staining of human kidney using 18135-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LYN Polyclonal antibody (18135-1-AP)

    IHC staining of human kidney using 18135-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using LYN Polyclonal antibody (18135-1-AP)

    IHC staining of human hepatocirrhosis using 18135-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using LYN Polyclonal antibody (18135-1-AP)

    IHC staining of human hepatocirrhosis using 18135-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LYN Polyclonal antibody (18135-1-AP)

    IF Staining of HeLa using 18135-1-AP

    Immunofluorescent analysis of HeLa cells using 18135-1-AP (LYN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using LYN Polyclonal antibody (18135-1-AP)

    FC experiment of Jurkat using 18135-1-AP

    1X10^6 Jurkat cells were stained with 0.2ug LYN antibody (18135-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 18135-1-AP is LYN Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LYN fusion protein Ag12723
Full Name v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Calculated Molecular Weight 512 aa, 59 kDa
Observed Molecular Weight 53-56 kDa, 66-70 kDa
GenBank Accession NumberBC075002
Gene Symbol LYN
Gene ID (NCBI) 4067
RRIDAB_11042319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

Protocols

Product Specific Protocols
WB protocol for LYN antibody 18135-1-APDownload protocol
IHC protocol for LYN antibody 18135-1-APDownload protocol
IF protocol for LYN antibody 18135-1-APDownload protocol
IP protocol for LYN antibody 18135-1-APDownload protocol
FC protocol for LYN antibody 18135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

Immune Characteristics of LYN in Tumor Microenvironment of Gliomas.

Authors - Chonghua Jiang
View Article