LYN Polyclonal antibody

LYN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18135-1-AP

Synonyms

JTK8, LYN, Tyrosine protein kinase Lyn



Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18135-1-AP is LYN Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LYN fusion protein Ag12723
Full Name v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Calculated molecular weight 512 aa, 59 kDa
Observed molecular weight 53-56 kDa, 66-70 kDa
GenBank accession numberBC075002
Gene symbol LYN
Gene ID (NCBI) 4067
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

Protocols

Product Specific Protocols
WB protocol for LYN antibody 18135-1-APDownload protocol
IHC protocol for LYN antibody 18135-1-APDownload protocol
IF protocol for LYN antibody 18135-1-APDownload protocol
IP protocol for LYN antibody 18135-1-APDownload protocol
FC protocol for LYN antibody 18135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols