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MAGEB1 Polyclonal antibody

MAGEB1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12400-1-AP

Synonyms

Cancer/testis antigen 3.1, CT3.1, DAM10, MAGE B1 antigen, MAGE Xp, MAGE XP antigen, MAGEB1, MAGEL1, MAGEXP, melanoma antigen family B, 1, Melanoma associated antigen B1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    WB analysis of HepG2 using 12400-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12400-1-AP (MAGEB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    WB analysis of A375 using 12400-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12400-1-AP (MAGEB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    IP experiment of HepG2 using 12400-1-AP

    IP result of anti-MAGEB1 (IP:12400-1-AP, 4ug; Detection:12400-1-AP 1:2000) with HepG2 cells lysate 3360 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    IF Staining of HepG2 using 12400-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAGEB1 antibody (12400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEB1 Polyclonal antibody (12400-1-AP)

    IF Staining of A375 using 12400-1-AP

    Immunofluorescent analysis of A375 cells using 12400-1-AP (MAGEB1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, A375 cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells, A375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12400-1-AP targets MAGEB1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEB1 fusion protein Ag3094
Full Name melanoma antigen family B, 1
Calculated Molecular Weight 347 aa, 39 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC013772
Gene Symbol MAGEB1
Gene ID (NCBI) 4112
RRIDAB_2877854
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGEB1, also named as MAGEL1, MAGEXP, DAM10 and CT3.1, is a member of the MAGEB gene family. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. The MW of MAGEB1 is about 40-45kd.

Protocols

Product Specific Protocols
WB protocol for MAGEB1 antibody 12400-1-APDownload protocol
IF protocol for MAGEB1 antibody 12400-1-APDownload protocol
IP protocol for MAGEB1 antibody 12400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols