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MAGEC3 Polyclonal antibody

MAGEC3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21491-1-AP

Synonyms

Cancer/testis antigen 7.2, CT7.2, HCA2, MAGE C3, MAGE C3 antigen, MAGEC3, MAGEC4, melanoma antigen family C, 3, Melanoma associated antigen C3

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MAGEC3 Polyclonal antibody (21491-1-AP)

    WB analysis of A375 using 21491-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 21491-1-AP (MAGEC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using MAGEC3 Polyclonal antibody (21491-1-AP)

    WB analysis of mouse thymus using 21491-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21491-1-AP (MAGEC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MAGEC3 Polyclonal antibody (21491-1-AP)

    IHC staining of human liver cancer using 21491-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 21491-1-AP (MAGEC3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MAGEC3 Polyclonal antibody (21491-1-AP)

    IHC staining of human liver cancer using 21491-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 21491-1-AP (MAGEC3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MAGEC3 Polyclonal antibody (21491-1-AP)

    IF Staining of A375 using 21491-1-AP

    Immunofluorescent analysis of A375 cells using 21491-1-AP (MAGEC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, mouse thymus tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21491-1-AP is MAGEC3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEC3 fusion protein Ag15819
Full Name melanoma antigen family C, 3
Calculated Molecular Weight 643 aa, 72 kDa
Observed Molecular Weight 70-72 kDa
GenBank Accession NumberBC103893
Gene Symbol MAGEC3
Gene ID (NCBI) 139081
RRIDAB_10734430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAGEC3 antibody 21491-1-APDownload protocol
IHC protocol for MAGEC3 antibody 21491-1-APDownload protocol
IF protocol for MAGEC3 antibody 21491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Cancer Commun (Lond)

MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.

Authors - Qingnan Wu
View Article
humanWB

BMC Cancer

Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells.

Authors - Xiao Song
View Article