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MAOA Polyclonal antibody

MAOA Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10539-1-AP

Synonyms

MAO A, MAOA, monoamine oxidase A, Monoamine oxidase type A

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using MAOA Polyclonal antibody (10539-1-AP)

    WB analysis of LNCaP using 10539-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MAOA Polyclonal antibody (10539-1-AP)

    WB analysis of human placenta using 10539-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MAOA Polyclonal antibody (10539-1-AP)

    WB analysis of human placenta using 10539-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human placenta tissue using MAOA Polyclonal antibody (10539-1-AP)

    IP experiment of human placenta using 10539-1-AP

    IP result of anti-MAOA (IP:10539-1-AP, 3ug; Detection:10539-1-AP 1:600) with human placenta tissue lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas cancer using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas cancer using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas cancer using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human kidney using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human kidney using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human liver using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human liver using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MAOA Polyclonal antibody (10539-1-AP)

    IHC staining of human pancreas cancer using 10539-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, human placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman pancreas cancer tissue, human pancreas tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10539-1-AP targets MAOA in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAOA fusion protein Ag0821
Full Name monoamine oxidase A
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC008064
Gene Symbol MAOA
Gene ID (NCBI) 4128
RRIDAB_2137251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAOA belongs to the flavin monoamine oxidase family. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

Protocols

Product Specific Protocols
WB protocol for MAOA antibody 10539-1-APDownload protocol
IHC protocol for MAOA antibody 10539-1-APDownload protocol
IP protocol for MAOA antibody 10539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

In situ identification of cellular drug targets in mammalian tissue.

Authors - Zhengyuan Pang
View Article
humanWB

Sci Adv

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Authors - Xiaochao Tan
View Article
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
View Article

J Clin Invest

A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.

Authors - Xiaochao Tan
View Article
humanWB,IHC

J Hepatol

Monoamine oxidase A suppresses hepatocellular carcinoma metastasis by inhibiting the adrenergic system and its transactivation of EGFR signaling.

Authors - Jun Li
View Article
mouseWB

Aging (Albany NY)

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Authors - Xiao Wu
View Article