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Animal-free recombinant proteins
Cell Division and Proliferation
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Con: 31 μg/150 μl
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human kidney tissue were subjected to SDS PAGE followed by western blot with 12745-1-AP(MEK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12745-1-AP( MEK6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12745-1-AP( MEK6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
1X10^6 MCF-7 cells were stained with 0.2ug MEK6 antibody (12745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
WB protocol for MEK6 antibody 12745-1-AP
IHC protocol for MEK6 antibody 12745-1-AP
FC protocol for MEK6 antibody 12745-1-AP
Standard WB protocol
Standard IHC protocol