MEK6 Polyclonal antibody

MEK6 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 12745-1-AP

Synonyms

MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, PRKMK6, SAPKK3



Tested Applications

Positive WB detected inHeLa cells, human kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12745-1-AP targets MEK6 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEK6 fusion protein Ag3706
Full Name mitogen-activated protein kinase kinase 6
Calculated Molecular Weight 334 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC012009
Gene Symbol MAP2K6
Gene ID (NCBI) 5608
RRIDAB_2141563
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MEK6 antibody 12745-1-APDownload protocol
IHC protocol for MEK6 antibody 12745-1-APDownload protocol
FC protocol for MEK6 antibody 12745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Food Funct

L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.

Authors - Kehong Liu
humanWB

Sci Rep

The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.

Authors - Leonardo Pedrazza
WB

Nat Aging

Skin chronological aging drives age-related bone loss via secretion of cystatin-A

Authors - Wenquan Liang
humanWB

Acta Pharmacol Sin

Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway

Authors - Ji-Xian Xiong
  • KD Validated