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MEKK2 Polyclonal antibody

MEKK2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 55106-1-AP

Synonyms

MAP3K2, MAPK/ERK kinase kinase 2, MAPKKK2, MEK kinase 2, MEKK 2, MEKK2, MEKK2B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MEKK2 Polyclonal antibody (55106-1-AP)

    WB analysis using 55106-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55106-1-AP (MEKK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MEKK2 Polyclonal antibody (55106-1-AP)

    IP experiment of HEK-293 using 55106-1-AP

    IP result of anti-MEKK2 (IP:55106-1-AP, 4ug; Detection:55106-1-AP 1:300) with HEK-293 cells lysate 3200ug.

Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55106-1-AP targets MEKK2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase kinase kinase 2
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession NumberNM_006609
Gene Symbol MAP3K2
Gene ID (NCBI) 10746
RRIDAB_11064604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K2, also named as MEKK2 and MEKK2B, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAPK kinase kinase subfamily. It is a component of a protein kinase signal transduction cascade. MAP3K2 mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. The antibody is specific to MAP3K2.

Protocols

Product Specific Protocols
WB protocol for MEKK2 antibody 55106-1-APDownload protocol
IP protocol for MEKK2 antibody 55106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP,WB

Mol Cancer

A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer

Authors - Runjie Song
View Article