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MAP4K1 Polyclonal antibody

MAP4K1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23950-1-AP

Synonyms

HPK1, MAP4K1, MEK kinase kinase 1, MEKKK 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using MAP4K1 Polyclonal antibody (23950-1-AP)

    WB analysis of Raji using 23950-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAP4K1 Polyclonal antibody (23950-1-AP)

    WB analysis using 23950-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MAP4K1 Polyclonal antibody (23950-1-AP)

    WB analysis of Jurkat using 23950-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MAP4K1 Polyclonal antibody (23950-1-AP)

    WB analysis of Jurkat using 23950-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using MAP4K1 Polyclonal antibody (23950-1-AP)

    IP experiment of Jurkat using 23950-1-AP

    IP result of anti-MAP4K1 (IP:23950-1-AP, 4ug; Detection:23950-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MAP4K1 Polyclonal antibody (23950-1-AP)

    IHC staining of human tonsillitis using 23950-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MAP4K1 Polyclonal antibody (23950-1-AP)

    IHC staining of human tonsillitis using 23950-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MAP4K1 Polyclonal antibody (23950-1-AP)

    IHC staining of human liver using 23950-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MAP4K1 Polyclonal antibody (23950-1-AP)

    IHC staining of human liver using 23950-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, Raji cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

23950-1-AP targets MAP4K1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP4K1 fusion protein Ag20221
Full Name mitogen-activated protein kinase kinase kinase kinase 1
Calculated Molecular Weight 833 aa, 91 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC152994
Gene Symbol MAP4K1
Gene ID (NCBI) 11184
RRIDAB_2879372
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4K1(Mitogen-activated protein kinase kinase kinase kinase 1) is also named as MEKKK 1, HPK1 and belongs to the protein kinase superfamily. It may function as a novel tumor suppressor and its loss plays a critical role in pancreatic cancer. MAP4K1 regulates stress responses, proliferation, and apoptosis in hematopoietic cells. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MAP4K1 antibody 23950-1-APDownload protocol
IHC protocol for MAP4K1 antibody 23950-1-APDownload protocol
IP protocol for MAP4K1 antibody 23950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Signal

SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells.

Authors - Xunping Zhao
View Article
humanWB

Clin Exp Med

An HPK1 inhibitor enhanced the tumour response to anti-PD-1 immunotherapy in non-Hodgkin's lymphoma

Authors - Lin Yang
View Article
humanWB

Nat Commun

DNA framework-engineered chimeras platform enables selectively targeted protein degradation

Authors - Li Zhou
View Article