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  • KD/KO Validated

MAP4K4 Polyclonal antibody

MAP4K4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55247-1-AP

Synonyms

FLH21957, HGK, HPK/GCK like kinase HGK, KIAA0687, MAP4K4, MEK kinase kinase 4, MEKKK 4, Nck interacting kinase, NIK



Tested Applications

Positive WB detected inJurkat cells, PC-3 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skeletal muscle tissue, human placenta tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55247-1-AP targets MAP4K4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase kinase kinase kinase 4
Calculated Molecular Weight 151 kDa
Observed Molecular Weight 130-160 kDa
GenBank Accession NumberNM_004834
Gene Symbol MAP4K4
Gene ID (NCBI) 9448
RRIDAB_10836939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.

Protocols

Product Specific Protocols
WB protocol for MAP4K4 antibody 55247-1-APDownload protocol
IHC protocol for MAP4K4 antibody 55247-1-APDownload protocol
IP protocol for MAP4K4 antibody 55247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Anal Chem

Parallel-Reaction-Monitoring-Based Proteome-Wide Profiling of Differential Kinase Protein Expression during Prostate Cancer Metastasis in Vitro.

Authors - Weili Miao
humanWB

EBioMedicine

Targeting miR-30d reverses pathological cardiac hypertrophy.

Authors - Jin Li
mouseWB

J Proteome Res

Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.

Authors - Jingzi Zhang
mouseWB,IF

Neural Regen Res

MAP4K4 induces early blood-brain barrier damage in a murine subarachnoid hemorrhage model.

Authors - Zheng Zou
  • KD Validated
humanIHC

PeerJ

Construction and validation of a novel prognostic signature of microRNAs in lung adenocarcinoma.

Authors - Wanzhen Li
humanWB

Pathol Res Pract

MiR-181c suppresses triple-negative breast cancer tumorigenesis by targeting MAP4K4.

Authors - Dan Xie