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JNK Polyclonal antibody

JNK Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17337-1-AP

Synonyms

c Jun N terminal kinase 2, JNK 55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, MAP kinase 9, MAPK 9, MAPK9, p54a, p54aSAPK, PRKM9, SAPK

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using JNK Polyclonal antibody (17337-1-AP)

    WB analysis of mouse brain using 17337-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17337-1-AP (JNK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using JNK Polyclonal antibody (17337-1-AP)

    IF Staining of SH-SY5Y using 17337-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 17337-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17337-1-AP targets JNK in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JNK fusion protein Ag11372
Full Name mitogen-activated protein kinase 9
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC032539
Gene Symbol MAPK9
Gene ID (NCBI) 5601
RRIDAB_2878388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Jun N-terminal kinases (JNKs), first characterized as stress-activated members of the mitogen-activated protein kinase (MAPK) family, have shown their critical roles in the development of a number of cellular progress and diseases, including proliferation, embryonic development, apoptosis, diabetes, neurodegeneration and liver disease (PMID: 19900593). JNK1, JNK2, JNK3 also name as MAPK8, MAPK9, MAPK10, JNK1 and JNK2 are ubiquitously expressed, but JNK3 is expressed primarily in the brain (PMID: 27698029). This antibody can recognize JNK1, JNK2 and JNK3, we get bands around 44-55 kDa.

Protocols

Product Specific Protocols
WB protocol for JNK antibody 17337-1-APDownload protocol
IF protocol for JNK antibody 17337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols