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MC1R Polyclonal antibody

MC1R Polyclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26471-1-AP

Synonyms

MC1 R, MC1R, Melanocortin receptor 1, MSH R, MSHR, SHEP2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using MC1R Polyclonal antibody (26471-1-AP)

    IHC staining of human brain using 26471-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26471-1-AP (MC1R Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MC1R Polyclonal antibody (26471-1-AP)

    IHC staining of human brain using 26471-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26471-1-AP (MC1R Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using MC1R Polyclonal antibody (26471-1-AP)

    IF Staining of A375 using 26471-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A375 cells using 26471-1-AP (MC1R antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A375 cells using MC1R Polyclonal antibody (26471-1-AP)

    FC experiment of A375 using 26471-1-AP

    1X10^6 A375 cells were intracellularly stained with 0.5 ug Anti-Human MC1R (26471-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells
Positive FC detected inA375 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26471-1-AP targets MC1R in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MC1R fusion protein Ag24006
Full Name melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
Calculated Molecular Weight 35 kDa
GenBank Accession NumberBC080622
Gene Symbol MC1R
Gene ID (NCBI) 4157
RRIDAB_2880529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MC1R antibody 26471-1-APDownload protocol
IF protocol for MC1R antibody 26471-1-APDownload protocol
FC protocol for MC1R antibody 26471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols