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MCCC1 Polyclonal antibody

MCCC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14861-1-AP

Synonyms

MCC B, MCCA, MCCase subunit alpha, MCCC1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using MCCC1 Polyclonal antibody (14861-1-AP)

    WB analysis of mouse liver using 14861-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14861-1-AP (MCCC1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MCCC1 Polyclonal antibody (14861-1-AP)

    WB analysis of mouse liver using 14861-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14861-1-AP (MCCC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using MCCC1 Polyclonal antibody (14861-1-AP)

    WB analysis of mouse heart using 14861-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14861-1-AP (MCCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using MCCC1 Polyclonal antibody (14861-1-AP)

    IP experiment of mouse liver using 14861-1-AP

    IP result of anti-MCCC1 (IP:14861-1-AP, 4ug; Detection:14861-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MCCC1 Polyclonal antibody (14861-1-AP)

    IHC staining of human liver cancer using 14861-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14861-1-AP (MCCC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MCCC1 Polyclonal antibody (14861-1-AP)

    IHC staining of human liver cancer using 14861-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14861-1-AP (MCCC1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse liver tissue, mouse heart tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14861-1-AP targets MCCC1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCCC1 fusion protein Ag6663
Full Name methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 70-76 kDa
GenBank Accession NumberBC004187
Gene Symbol MCCC1
Gene ID (NCBI) 56922
RRIDAB_2143798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCCC1 is the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Protocols

Product Specific Protocols
WB protocol for MCCC1 antibody 14861-1-APDownload protocol
IHC protocol for MCCC1 antibody 14861-1-APDownload protocol
IP protocol for MCCC1 antibody 14861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biology (Basel)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Authors - Olga Gourdomichali
View Article
humanWB,IHC

Life Sci

Methylcrotonyl-CoA carboxylase subunit 1 (MCCA) regulates multidrug resistance in multiple myeloma

Authors - Yu Feng
View Article