MCM5 Monoclonal antibody

MCM5 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3B7F8

Cat no : 67049-1-Ig

Synonyms

CDC46, CDC46 homolog, MCM5, P1 CDC46



Tested Applications

Positive WB detected inJurkat cells, A375 cells, HeLa cells, ROS1728 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67049-1-Ig targets MCM5 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCM5 fusion protein Ag28704
Full Name minichromosome maintenance complex component 5
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 82-88 kDa
GenBank Accession NumberBC000142
Gene Symbol MCM5
Gene ID (NCBI) 4174
RRIDAB_2882362
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

Protocols

Product Specific Protocols
WB protocol for MCM5 antibody 67049-1-IgDownload protocol
IF protocol for MCM5 antibody 67049-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Ther

Long noncoding RNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5.

Authors - Yingying Jiang
mouseWB

Nat Cancer

Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis.

Authors - Minhong Shen