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MCM7 Monoclonal antibody

MCM7 Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1F6

Cat no : 67446-1-Ig

Synonyms

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM7 Monoclonal antibody (67446-1-Ig)

    WB analysis using 67446-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67446-1-Ig (MCM7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MCM7 Monoclonal antibody (67446-1-Ig)

    IP experiment of HeLa using 67446-1-Ig

    IP result of anti-MCM7 (IP:67446-1-Ig, 4ug; Detection:67446-1-Ig 1:2000) with HeLa cells lysate 1040 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MCM7 Monoclonal antibody (67446-1-Ig)

    IHC staining of human breast cancer using 67446-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MCM7 Monoclonal antibody (67446-1-Ig)

    IHC staining of human lung cancer using 67446-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MCM7 Monoclonal antibody (67446-1-Ig)

    IHC staining of human lung cancer using 67446-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MCM7 Monoclonal antibody (67446-1-Ig)

    IHC staining of human breast cancer using 67446-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67446-1-Ig targets MCM7 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCM7 fusion protein Ag28558
Full Name minichromosome maintenance complex component 7
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC013375
Gene Symbol MCM7
Gene ID (NCBI) 4176
RRIDAB_2882680
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2–7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

Protocols

Product Specific Protocols
WB protocol for MCM7 antibody 67446-1-IgDownload protocol
IHC protocol for MCM7 antibody 67446-1-IgDownload protocol
IP protocol for MCM7 antibody 67446-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols