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MCOLN1 Polyclonal antibody

MCOLN1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15291-1-AP

Synonyms

MCOLN1, MG 2, ML4, MLIV, MST080, MSTP080, Mucolipidin, mucolipin 1, TRP ML1, TRPM L1, TRPML1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCOLN1 Polyclonal antibody (15291-1-AP)

    WB analysis using 15291-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15291-1-AP (MCOLN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MCOLN1 Polyclonal antibody (15291-1-AP)

    IF Staining of U2OS using 15291-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using MCOLN1 antibody (15291-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue, mouse kidney tissue, rat kidney tissue
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15291-1-AP targets MCOLN1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCOLN1 fusion protein Ag7493
Full Name mucolipin 1
Calculated Molecular Weight 65 kDa
Observed Molecular Weight60-65 kDa
GenBank Accession NumberBC005149
Gene Symbol MCOLN1
Gene ID (NCBI) 57192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCOLN1, also named MLIV, ML4, MG-2, and Mucolipidin, belongs to the transient receptor (TC 1.A.4) family, Polycystin subfamily, and MCOLN1 sub-subfamily. It is playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. MCOLN1 may play a major role in Ca2+ transport regulating lysosomal exocytosis. It is an autosomal recessive lysosomal storage disorder characterized by severe psychomotor retardation and ophthalmologic abnormalities, including corneal opacity, retinal degeneration, and strabismus.

Protocols

Product Specific Protocols
WB protocol for MCOLN1 antibody 15291-1-APDownload protocol
IF protocol for MCOLN1 antibody 15291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
View Article