- Featured Product
- KD/KO Validated
MED30 Polyclonal antibody
MED30 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16787-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | human heart tissue, HEK-293 cells |
| Positive IP detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 4 publications below |
Product Information
16787-1-AP targets MED30 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MED30 fusion protein Ag10221 |
| Full Name | mediator complex subunit 30 |
| Calculated Molecular Weight | 178 aa, 20 kDa |
| Observed Molecular Weight | 20-25 kDa |
| GenBank Accession Number | BC008226 |
| Gene Symbol | MED30 |
| Gene ID (NCBI) | 90390 |
| RRID | AB_2142439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED30 antibody 16787-1-AP | Download protocol |
| IP protocol for MED30 antibody 16787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Autoimmun Immune complexome analysis of a rich variety of serum immune complexes identifies disease-characteristic immune complex antigens in systemic sclerosis | ||
Anticancer Res The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer.
| ||
Ann Diagn Pathol The knockdown of the mediator complex subunit MED30 suppresses the proliferation and migration of renal cell carcinoma cells.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tongbin (Verified Customer) (08-25-2020) | This antibody detects a MED30 band at around 22kD.
|
