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MED8 Polyclonal antibody

MED8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12182-1-AP

Synonyms

ARC32, MED8, mediator complex subunit 8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using MED8 Polyclonal antibody (12182-1-AP)

    WB analysis of HepG2 using 12182-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MED8 Polyclonal antibody (12182-1-AP)

    WB analysis of mouse liver using 12182-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using MED8 Polyclonal antibody (12182-1-AP)

    WB analysis of SGC-7901 using 12182-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12182-1-AP (MED8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using MED8 Polyclonal antibody (12182-1-AP)

    IHC staining of human brain using 12182-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using MED8 Polyclonal antibody (12182-1-AP)

    IHC staining of human brain using 12182-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12182-1-AP (MED8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, SGC-7901 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12182-1-AP targets MED8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MED8 fusion protein Ag2827
Full Name mediator complex subunit 8
Calculated Molecular Weight 212 aa, 23 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC010543
Gene Symbol MED8
Gene ID (NCBI) 112950
RRIDAB_2877834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.

Protocols

Product Specific Protocols
WB protocol for MED8 antibody 12182-1-APDownload protocol
IHC protocol for MED8 antibody 12182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.

Authors - Wen-Shuai Tang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

siting (Verified Customer) (06-24-2021)

clear band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mice heart
MED8 Antibody Western Blot validation (1:500 dilution) in mice heart (Cat no:12182-1-AP)
FH

SITING (Verified Customer) (05-25-2021)

GOOD, SOMETIME WILL show some unspecific bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
MED8 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:12182-1-AP)