MEF2C Polyclonal antibody
MEF2C Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 18290-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
18290-1-AP targets MEF2C in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | myocyte enhancer factor 2C |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 45-70 kDa |
| GenBank Accession Number | NM_002397 |
| Gene Symbol | MEF2C |
| Gene ID (NCBI) | 4208 |
| RRID | AB_2142849 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MEF2C antibody 18290-1-AP | Download protocol |
| IHC protocol for MEF2C antibody 18290-1-AP | Download protocol |
| IF protocol for MEF2C antibody 18290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature Genetic and epigenetic coordination of cortical interneuron development.
| ||
Biol Reprod Integrated microRNA and mRNA network analysis of the human myometrial transcriptome in the transition from quiescence to labor. | ||
Cell Rep Chromatin Environment and Cellular Context Specify Compensatory Activity of Paralogous MEF2 Transcription Factors. | ||
Cell Genom Single nucleus multiomics identifies ZEB1 and MAFB as candidate regulators of Alzheimer's disease-specific cis-regulatory elements |
