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MIER1 Polyclonal antibody

MIER1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11452-1-AP

Synonyms

Early response 1, ER1, hMI ER1, KIAA1610, MI ER1, MIER1, RP5 944N15.1

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using MIER1 Polyclonal antibody (11452-1-AP)

    WB analysis of human testis using 11452-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MIER1 Polyclonal antibody (11452-1-AP)

    WB analysis of HepG2 using 11452-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MIER1 Polyclonal antibody (11452-1-AP)

    WB analysis of HepG2 using 11452-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using MIER1 Polyclonal antibody (11452-1-AP)

    WB analysis of mouse heart using 11452-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11452-1-AP (MIER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MIER1 Polyclonal antibody (11452-1-AP)

    IHC staining of human pancreas cancer using 11452-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MIER1 Polyclonal antibody (11452-1-AP)

    IHC staining of human pancreas cancer using 11452-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11452-1-AP (MIER1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MIER1 Polyclonal antibody (11452-1-AP)

    IHC staining of human pancreas cancer using 11452-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11452-1-AP (MIER1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MIER1 Polyclonal antibody (11452-1-AP)

    IF Staining of HepG2 using 11452-1-AP

    Immunofluorescent analysis of HepG2 cells using 11452-1-AP (MIER1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman testis tissue, HepG2 cells, mouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11452-1-AP targets MIER1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIER1 fusion protein Ag2010
Full Name mesoderm induction early response 1 homolog (Xenopus laevis)
Calculated Molecular Weight 536 aa, 61 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC017423
Gene Symbol MIER1
Gene ID (NCBI) 57708
RRIDAB_10733247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MIER1 antibody 11452-1-APDownload protocol
IHC protocol for MIER1 antibody 11452-1-APDownload protocol
IF protocol for MIER1 antibody 11452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,ChIP

Gastroenterology

Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors

Authors - Mingjun Sun
View Article
mouseIHC

Nat Commun

Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice

Authors - Yanhao Chen
View Article
humanWB

Cell Chem Biol

HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation

Authors - Yufeng Xiao
View Article