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MIER3 Polyclonal antibody

MIER3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17543-1-AP

Synonyms

Mi er3, MIER3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse uterus tissue using MIER3 Polyclonal antibody (17543-1-AP)

    WB analysis of mouse uterus using 17543-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17543-1-AP (MIER3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse uterus tissue using MIER3 Polyclonal antibody (17543-1-AP)

    WB analysis of mouse uterus using 17543-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17543-1-AP (MIER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse uterus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17543-1-AP targets MIER3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIER3 fusion protein Ag11604
Full Name mesoderm induction early response 1, family member 3
Calculated Molecular Weight 522 aa, 59 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC041348
Gene Symbol MIER3
Gene ID (NCBI) 166968
RRIDAB_2143938
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mesoderm induction early response (MIER) protein family is known as fibroblast growth factor (FGF)-regulated immediate-early protein family. Activated by FGF, MIER proteins may have a pivotal role in FGF-regulated cellular activities, indicating a potential influence in the progression of certain cancers [PMID:15117948]. MIER3 localizes to the nucleus and primarily functions as a transcriptional repressor. Rat mammary carcinoma susceptibility 1b (Mcs1b) is a quantitative trait locus on RN02 that confers decreased susceptibility when Copenhagen (COP)-resistant alleles are introgressed into a Wistar Furth (WF)-susceptible genome. MIER3 is a candidate Mcs1b gene [PMID:22993404].

Protocols

Product Specific Protocols
WB protocol for MIER3 antibody 17543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mitochondrion

Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5.

Authors - Shih-Wei Chen
View Article