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MIOS Polyclonal antibody

MIOS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20826-1-AP

Synonyms

MIOS

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using MIOS Polyclonal antibody (20826-1-AP)

    WB analysis of K-562 using 20826-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MIOS Polyclonal antibody (20826-1-AP)

    WB analysis of HeLa using 20826-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using MIOS Polyclonal antibody (20826-1-AP)

    WB analysis of rat liver using 20826-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using MIOS Polyclonal antibody (20826-1-AP)

    WB analysis of PC-3 using 20826-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20826-1-AP (MIOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MIOS Polyclonal antibody (20826-1-AP)

    IP experiment of HeLa using 20826-1-AP

    IP result of anti-MIOS (IP:20826-1-AP, 4ug; Detection:20826-1-AP 1:300) with HeLa cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MIOS Polyclonal antibody (20826-1-AP)

    IHC staining of mouse skeletal muscle using 20826-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MIOS Polyclonal antibody (20826-1-AP)

    IHC staining of mouse skeletal muscle using 20826-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MIOS Polyclonal antibody (20826-1-AP)

    IHC staining of mouse skeletal muscle using 20826-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MIOS Polyclonal antibody (20826-1-AP)

    IHC staining of mouse skeletal muscle using 20826-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20826-1-AP (MIOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, rat liver tissue, HeLa cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20826-1-AP targets MIOS in WB, IP, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIOS fusion protein Ag14802
Full Name missing oocyte, meiosis regulator, homolog (Drosophila)
Calculated Molecular Weight 875 aa, 99 kDa
Observed Molecular Weight 99 kDa
GenBank Accession NumberBC005883
Gene Symbol MIOS
Gene ID (NCBI) 54468
RRIDAB_2878747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MIOS antibody 20826-1-APDownload protocol
IHC protocol for MIOS antibody 20826-1-APDownload protocol
IP protocol for MIOS antibody 20826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Am J Physiol Endocrinol Metab

Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model.

Authors - Marko Rudar
View Article
humanWB

JCI Insight

GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion positive rhabdomyosarcoma

Authors - Jacqueline Morales
  • KD Validated
View Article