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  • Featured Product
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MLN64 Polyclonal antibody

MLN64 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20292-1-AP

Synonyms

CAB1, MLN 64, MLN64, Protein CAB1, STARD3

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using MLN64 Polyclonal antibody (20292-1-AP)

    WB analysis of MCF-7 using 20292-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MLN64 Polyclonal antibody (20292-1-AP)

    WB analysis of Jurkat using 20292-1-AP

    WB result of MLN64 antibody (20292-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLN64 transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using MLN64 Polyclonal antibody (20292-1-AP)

    WB analysis using 20292-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using MLN64 Polyclonal antibody (20292-1-AP)

    WB analysis of mouse placenta using 20292-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using MLN64 Polyclonal antibody (20292-1-AP)

    IP experiment of MCF-7 using 20292-1-AP

    IP result of anti-MLN64 (IP:20292-1-AP, 3ug; Detection:20292-1-AP 1:500) with MCF-7 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MLN64 Polyclonal antibody (20292-1-AP)

    IHC staining of human ovary tumor using 20292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MLN64 Polyclonal antibody (20292-1-AP)

    IHC staining of human ovary tumor using 20292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MLN64 Polyclonal antibody (20292-1-AP)

    IF Staining of MCF-7 using 20292-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20292-1-AP (MLN64 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, mouse placenta tissue, MCF-7 cells, A549 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20292-1-AP is MLN64 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLN64 fusion protein Ag14061
Full Name StAR-related lipid transfer (START) domain containing 3
Calculated Molecular Weight 445 aa, 51 kDa
Observed Molecular Weight 33-53 kDa
GenBank Accession NumberBC008747
Gene Symbol STARD3
Gene ID (NCBI) 10948
RRIDAB_10643978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLN64 (also known as STARD3) is an integral membrane protein anchored in late endosomes. MLN64 has a StAR-related lipid-transfer (START) domain to bind cholesterol, mediating intracellular cholesterol transfer. MLN64 is widely expressed in multiple tissues including liver, spleen, heart, kidney, lung, and the brain. Higher expression of MLN64 has been found in several malignancies. MLN64 can be detected as 33 kDa truncated fragment (PMID:9237999, PMID:15718238).

Protocols

Product Specific Protocols
WB protocol for MLN64 antibody 20292-1-APDownload protocol
IHC protocol for MLN64 antibody 20292-1-APDownload protocol
IF protocol for MLN64 antibody 20292-1-APDownload protocol
IP protocol for MLN64 antibody 20292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

PMID: 24905460

Authors - Rui Liu
View Article