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MMADHC Polyclonal antibody

MMADHC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 23191-1-AP

Synonyms

C2orf25, cblD, CL25022, MMADHC, My011

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMADHC Polyclonal antibody (23191-1-AP)

    WB analysis using 23191-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MMADHC Polyclonal antibody (23191-1-AP)

    WB analysis of HeLa using 23191-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MMADHC Polyclonal antibody (23191-1-AP)

    WB analysis of MCF-7 using 23191-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MMADHC Polyclonal antibody (23191-1-AP)

    IHC staining of human skeletal muscle using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MMADHC Polyclonal antibody (23191-1-AP)

    IHC staining of human skeletal muscle using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MMADHC Polyclonal antibody (23191-1-AP)

    IHC staining of human heart using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 23191-1-AP (MMADHC antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MMADHC Polyclonal antibody (23191-1-AP)

    IHC staining of human heart using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 23191-1-AP (MMADHC antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MMADHC Polyclonal antibody (23191-1-AP)

    IHC staining of human heart using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MMADHC Polyclonal antibody (23191-1-AP)

    IHC staining of human heart using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using MMADHC Polyclonal antibody (23191-1-AP)

    IHC staining of human pancreas using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using MMADHC Polyclonal antibody (23191-1-AP)

    IHC staining of human pancreas using 23191-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, NIH3T3 cells
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23191-1-AP is MMADHC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMADHC fusion protein Ag19343
Full Name methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria
Calculated Molecular Weight 296 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC022859
Gene Symbol MMADHC
Gene ID (NCBI) 27249
RRIDAB_2879229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMADHC is a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. MMADHC is a 296-amino acids (32.9 kDa) protein with an N-terminal disordered region (amino acids 1-107) containing a potential mitochondrial leader sequence (MLS; amino acids 1-12), and a C-terminal Nitro Reductase-like domain (NTR; amino acids 108-296). Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin.

Protocols

Product Specific Protocols
WB protocol for MMADHC antibody 23191-1-APDownload protocol
IHC protocol for MMADHC antibody 23191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols