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  • Featured Product
  • KD/KO Validated

MOF Polyclonal antibody

MOF Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 13842-1-AP

Synonyms

hMOF, KAT8, MOF, MYST 1, MYST1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MOF Polyclonal antibody (13842-1-AP)

    WB analysis of HeLa using 13842-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MOF Polyclonal antibody (13842-1-AP)

    WB analysis of MCF-7 using 13842-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using MOF Polyclonal antibody (13842-1-AP)

    WB analysis of rat liver using 13842-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MOF Polyclonal antibody (13842-1-AP)

    WB analysis of MCF-7 using 13842-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MOF Polyclonal antibody (13842-1-AP)

    WB analysis of MCF-7 using 13842-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MOF Polyclonal antibody (13842-1-AP)

    WB analysis of HeLa using 13842-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MOF Polyclonal antibody (13842-1-AP)

    WB analysis of HeLa using 13842-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MOF Polyclonal antibody (13842-1-AP)

    WB analysis of HeLa using 13842-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13842-1-AP (MOF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MOF Polyclonal antibody (13842-1-AP)

    IP experiment of HeLa using 13842-1-AP

    IP result of anti-MOF (IP:13842-1-AP, 4ug; Detection:13842-1-AP 1:600) with HeLa cells lysate 1200ug.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, rat liver tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13842-1-AP targets MOF in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOF fusion protein Ag4802
Full Name MYST histone acetyltransferase 1
Calculated Molecular Weight 467 aa, 53 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC037773
Gene Symbol MYST1
Gene ID (NCBI) 84148
RRIDAB_2146894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOF, also known as MYST1, KAT8, is a member of the MYST acetyltransferase family, which is homologous to Drosophila males absent on the first (MOF) protein. Deletion of MYST1 in cells leads to genomic instability, spontaneous chromosomal aberration, cell cycle defects, reduced transcription of certain genes and defective DNA damage repair (PMID: 32711345). MYST1 dually localizes to the nucleus and mitochondria of human cancer cells (PMID: 37813994). Western blot analysis detected MOF at an apparent molecular mass of 52 kDa.

Protocols

Product Specific Protocols
WB protocol for MOF antibody 13842-1-APDownload protocol
IP protocol for MOF antibody 13842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A druggable copper-signalling pathway that drives inflammation

Authors - Stéphanie Solier
View Article
humanWB,IP

Autophagy

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.

Authors - Guokai Yan
View Article
humanWB,ChIP

Redox Biol

Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.

Authors - Ming Kong
  • KD Validated
View Article
humanWB

Mol Cell Proteomics

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

Authors - Heguo Yu
View Article
humanWB

Mol Cell Proteomics

Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.

Authors - Ding Wang
View Article
mouseWB

Cell Physiol Biochem

Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis.

Authors - Yang Yang
  • KO Validated
View Article