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MR1 Polyclonal antibody

MR1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IF,ELISA

Conjugate

Unconjugated

Cat no : 13260-1-AP

Synonyms

HLALS, MR1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of THP-1 cells using MR1 Polyclonal antibody (13260-1-AP)

    WB analysis of THP-1 cells using 13260-1-AP

    WB result of MR1 antibody (13260-1-AP, 1:250) with IP samples of THP.1-MR1 cells, by Claudia Gonzalez-Lopez, MRC Human Immunology Unit, University of Oxford. (Green MR1 protein).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using MR1 Polyclonal antibody (13260-1-AP)

    IF Staining of THP-1 cells using 13260-1-AP

    IF result of MR1 antibody (13260-1-AP, 1:50) with THP.1-MR1 cells, by Claudia Gonzalez-Lopez, MRC Human Immunology Unit, University of Oxford.

Tested Applications

Positive WB detected inTHP-1 cells
Positive IF detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:100-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13260-1-AP targets MR1 in WB,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MR1 fusion protein Ag3849
Full Name major histocompatibility complex, class I-related
Calculated Molecular Weight 341 aa, 39 kDa
GenBank Accession NumberBC012485
Gene Symbol MR1
Gene ID (NCBI) 3140
RRIDAB_2877930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Publications

SpeciesApplicationTitle
humanWB,IF

Proc Natl Acad Sci U S A

Ligand-dependent downregulation of MR1 cell surface expression.

Authors - Mariolina Salio
View Article
mouseIF

Carbohydr Polym

Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy.

Authors - Tingting Guo
View Article
humanWB

J Biol Chem

The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation.

Authors - Corinna A Kulicke
View Article
humanFC

Am J Pathol

Bile from Patients with Primary Sclerosing Cholangitis Contains Mucosal-Associated Invariant T-Cell Antigens.

Authors - Laura Valestrand
View Article
mouseIF

Pigment Cell Melanoma Res

Topical antibiotics limit depigmentation in a mouse model of vitiligo

Authors - Ahmed Ahmed Touni
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tina (Verified Customer) (08-31-2020)

- PAGE was loaded with 80ug total protein - PVDF membrane blocked with milk - first AB dilution 1:250 (stored at -20°C, I could reuse the solution multiple times) - secondary AB I used HRP goat anti-rabbit and a high sensitive ECl to develop the Blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: cell lines K562, primary liver cells