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MRAP Polyclonal antibody

MRAP Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19512-1-AP

Synonyms

B27, C21orf61, FALP, FGD2, GCCD2, MRAP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using MRAP Polyclonal antibody (19512-1-AP)

    WB analysis of mouse kidney using 19512-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19512-1-AP (MRAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using MRAP Polyclonal antibody (19512-1-AP)

    IHC staining of human testis using 19512-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19512-1-AP (MRAP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MRAP Polyclonal antibody (19512-1-AP)

    IHC staining of human testis using 19512-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19512-1-AP (MRAP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MRAP Polyclonal antibody (19512-1-AP)

    IHC staining of mouse kidney using 19512-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney using 19512-1-AP (MRAP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MRAP Polyclonal antibody (19512-1-AP)

    IHC staining of mouse kidney using 19512-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney using 19512-1-AP (MRAP antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRAP Polyclonal antibody (19512-1-AP)

    IF Staining of HeLa using 19512-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19512-1-AP (MRAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19512-1-AP targets MRAP in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name melanocortin 2 receptor accessory protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberNM_178817
Gene Symbol MRAP
Gene ID (NCBI) 56246
RRIDAB_10694272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRAP, also named as C21orf61, FALP and B27, is required for MC2R expression in certain cell types. MRAP is involved in the processing, trafficking or function of MC2R. MRAP may be involved in the intracellular trafficking pathways in adipocyte cells. Mutation of MRAP will cause glucocorticoid deficiency type 2 (GCCD2) which known as familial glucocorticoid deficiency type 2 (FGD2). The antibody can recognize the isoform1,isoform2 and isoform4.

Protocols

Product Specific Protocols
WB protocol for MRAP antibody 19512-1-APDownload protocol
IHC protocol for MRAP antibody 19512-1-APDownload protocol
IF protocol for MRAP antibody 19512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Tissue Res

ACTH promotes chondrogenic nodule formation and induces transient elevations in intracellular calcium in rat bone marrow cell cultures via MC2-R signaling.

Authors - Jodi F Evans
View Article