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MRI1 Polyclonal antibody

MRI1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19916-1-AP

Synonyms

M1Pi, MRDI, MRI1, MTNA, MTR 1 P isomerase, Ypr118w

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using MRI1 Polyclonal antibody (19916-1-AP)

    WB analysis of HT-1080 using 19916-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using MRI1 Polyclonal antibody (19916-1-AP)

    WB analysis of HL-60 using 19916-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MRI1 Polyclonal antibody (19916-1-AP)

    IHC staining of rat kidney using 19916-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MRI1 Polyclonal antibody (19916-1-AP)

    IHC staining of rat kidney using 19916-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHT-1080 cells, HL-60 cells
Positive IHC detected inrat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19916-1-AP is MRI1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRI1 fusion protein Ag13809
Full Name methylthioribose-1-phosphate isomerase homolog (S. cerevisiae)
Calculated Molecular Weight 369 aa, 39 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC001703
Gene Symbol MRI1
Gene ID (NCBI) 84245
RRIDAB_10638924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRI1 antibody 19916-1-APDownload protocol
IHC protocol for MRI1 antibody 19916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols