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MRPS30 Polyclonal antibody

MRPS30 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 18441-1-AP

Synonyms

MRP S30, MRPS30, PAP, PDCD9, S30mt

Validation Data Gallery

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  • Western Blot (WB) analysis of human myotube protein using MRPS30 Polyclonal antibody (18441-1-AP)

    WB analysis of human myotube protein using 18441-1-AP

    WB result of MRPS30 antibody (18441-1-AP, 1:1000) with with 15µg of control human myotube protein by Max Pohjanpelto, University of Helsinki.

  • Western Blot (WB) analysis of HeLa cells using MRPS30 Polyclonal antibody (18441-1-AP)

    WB analysis of HeLa using 18441-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18441-1-AP (MRPS30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver mitochondria tissue using MRPS30 Polyclonal antibody (18441-1-AP)

    WB analysis of mouse liver mitochondria using 18441-1-AP

    mouse liver mitochondria tissue were subjected to SDS PAGE followed by western blot with 18441-1-AP (MRPS30 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPS30 Polyclonal antibody (18441-1-AP)

    IF Staining of HepG2 using 18441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18441-1-AP (MRPS30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman myotube protein, HeLa cells, mouse liver mitochondria tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18441-1-AP is MRPS30 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS30 fusion protein Ag13271
Full Name mitochondrial ribosomal protein S30
Calculated Molecular Weight 439 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC007735
Gene Symbol MRPS30
Gene ID (NCBI) 10884
RRIDAB_2878544
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPS30 antibody 18441-1-APDownload protocol
IF protocol for MRPS30 antibody 18441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols