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MRPS31 Polyclonal antibody

MRPS31 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16288-1-AP

Synonyms

Imogen 38, IMOGN38, MRP S31, MRPS31, S31mt

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MRPS31 Polyclonal antibody (16288-1-AP)

    WB analysis of HeLa using 16288-1-AP

    WB result of MRPS31 antibody (16288-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS31 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using MRPS31 Polyclonal antibody (16288-1-AP)

    WB analysis using 16288-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16288-1-AP (MRPS31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MRPS31 Polyclonal antibody (16288-1-AP)

    WB analysis using 16288-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16288-1-AP (MRPS31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPS31 Polyclonal antibody (16288-1-AP)

    IF Staining of HeLa using 16288-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPS31 antibody (16288-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, MCF-7 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16288-1-AP targets MRPS31 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS31 fusion protein Ag9434
Full Name mitochondrial ribosomal protein S31
Calculated Molecular Weight 395 aa, 45 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC022045
Gene Symbol MRPS31
Gene ID (NCBI) 10240
RRIDAB_2297732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPS31 antibody 16288-1-APDownload protocol
IF protocol for MRPS31 antibody 16288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.

Authors - Juliette Pulman
View Article