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MRRF Polyclonal antibody

MRRF Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12357-2-AP

Synonyms

MRFF, MRRF, MTRRF, RRF

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MRRF Polyclonal antibody (12357-2-AP)

    WB analysis using 12357-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12357-2-AP (MRRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MRRF Polyclonal antibody (12357-2-AP)

    IHC staining of human pancreas cancer using 12357-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12357-2-AP (MRRF antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MRRF Polyclonal antibody (12357-2-AP)

    IF Staining of MCF-7 using 12357-2-AP

    Immunofluorescent analysis of MCF-7 cells, using MRRF antibody 12357-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

12357-2-AP targets MRRF in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRRF fusion protein Ag3021
Full Name mitochondrial ribosome recycling factor
Calculated Molecular Weight 262 aa, 29 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC013049
Gene Symbol MRRF
Gene ID (NCBI) 92399
RRIDAB_2144259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRRF antibody 12357-2-APDownload protocol
IHC protocol for MRRF antibody 12357-2-APDownload protocol
IF protocol for MRRF antibody 12357-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.

Authors - Takumi Yokokawa
View Article
mouseWB

Physiol Rep

Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.

Authors - Takumi Yokokawa
View Article

EMBO J

Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism

Authors - Ying Shu
View Article
ratWB

Sci Total Environ

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects

Authors - Dongjie Li
View Article