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- KD/KO Validated
MTA3 Polyclonal antibody
MTA3 Polyclonal Antibody for WB,IP,IHC,IF,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB,IP,IF,IHC,ELISA
Conjugate
Unconjugated
Cat no : 14682-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse thymus tissue, Jurkat cells, MCF-7 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 8 publications below |
Product Information
14682-1-AP targets MTA3 in WB,IP,IF,IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MTA3 fusion protein Ag6400 |
| Full Name | metastasis associated 1 family, member 3 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 62 kDa, 68 kDa |
| GenBank Accession Number | BC053631 |
| Gene Symbol | MTA3 |
| Gene ID (NCBI) | 57504 |
| RRID | AB_2298003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTA3 antibody 14682-1-AP | Download protocol |
| IHC protocol for MTA3 antibody 14682-1-AP | Download protocol |
| IF protocol for MTA3 antibody 14682-1-AP | Download protocol |
| IP protocol for MTA3 antibody 14682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development. | ||
Mol Cell The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression. | ||
Oncotarget Metastasis-associated protein 3 in colorectal cancer determines tumor recurrence and prognosis. | ||
PLoS One Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer.
| ||
J Gastroenterol Hepatol Overexpression of the metastasis-associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma. | ||
Tumour Biol MTA3 regulates malignant progression of colorectal cancer through Wnt signaling pathway. |
